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So that you can reduce steadily the complexity of the plant herb to purify its phytochemical biomolecules, a novel fractionation method making use of different solvent combination ratios was used to separate twelve bioactive portions. These portions had been tested for activity in the biogenic synthesis of cobalt oxide nanoparticles, biofilm and antifungal tasks. The outcomes revealed that plant extract with bioactive fractions in 30% ratio for many solvent combinatio
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