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The prevailing strains, Gammaproteobacteria (35.5%) and Alphaproteobacteria (56.5%), played an important active role in organic carbon removal. The integrated system has great potential as pretreatment for shale gas wastewater due to its low energy consumption, low operational costs, high productivity, and effluent quality.Microplastics (MPS) are widespread in our environment and have a potential impact on the reproductive development of humans and mammals. In this study, we evaluated the effect of 5 µm polystyrene microplastics(PS-MPS) o