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Paromomycin adsorbs to glass containers at low concentrations, and therefore acidic conditions were used throughout the assay, in combination with polypropylene tubes and autosampler vials. The assay was successfully applied in a pharmacokinetic study in visceral leishmaniasis patients from Eastern Africa. Herbal medicine (HM) has been playing a pivotal role in maintaining human health since ancient times, and its therapeutic theory and clinical experience are the precious traditional medical knowledge reserves. As HM occupies an importa