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The transmembrane transport of higher concentrations of alanine and methionine in Group B was essential for cell metabolism and nutrient transport, thereby enriching nitrogen and sulfur-associated communities. In this investigation, insights into carbon, nitrogen and sulfur bioconversions in sewer ecosystems were revealed, significantly improving the understanding of the sewer ecosystem within a community context.Data assimilation (DA) techniques are powerful means of dynamic natural system modeling that allow for the use of data as soo