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One of the drawbacks of Ser222-Ala229-Cys330-BCaMP3 was that the fluorescence intensity was not enough when cells were cultured under 37°C. However, this limitation was circumvented by lowering cell culture temperature to 28°C, allowing much more enhanced blue fluorescence. Although Ser222-Ala229-Cys330-BCaMP3 mandates further optimization, the present study has found a promising blue fluorescent GECI that is derived from GCaMP3.(Z)-2-(4-methoxybenzylideneamino)-3-phenylpropanoic acid (L) synthesized by condensation of p-anisaldehyde and
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