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Portal vein (PV) embolization is performed prior to extended hepatectomy for the damaged liver to increase future remnant liver volume and prevent postoperative liver failure. This study examined whether two-stage PV ligation (PVL) increased regeneration and hypertrophy of the future remnant liver compared to conventional PVL, and whether two-stage PVL was safe for damaged liver. We produced a cirrhotic liver rat model with perioperatively maintained fibrosis. Rats were divided into Group A (70%PVL), ligation of left branch of PV; Group
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