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This study demonstrated that pure biofilms without containing inorganic particles were ideal materials for DMs, which could achieve long-term stable operation with low effluent turbidity ( less then 1 NTU) and low TMP ( less then 10 Pa), while inorganic particles with any size could deteriorate the filtration performance. Therefore, removing the inorganic particles in wastewater as many as possible prior DMBR is critically important for achieving long-term stable operation.In the semi-arid high mountains of the Upper Indus Basin (UI,