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We highlight long-standing questions in each of these areas that cryo-EM can potentially address, which would firmly establish the powerful tool's broad scope and utility beyond biology.Local chemotherapy is a clinically proven strategy in treating malignant brain tumors. Its benefits, however, are largely limited by the rapid release and clearance of therapeutic agents and the inability to penetrate through tumor tissues. We report here on a supramolecular tubustecan (TT) hydrogel as both a therapeutic and drug carrier that enables long-