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Although many alginate lyases have been characterized, little is yet understood about those in excessively cold polar environments, which may have unique mechanisms for environmental adaptation as well as for alginate degradation. Here, we report the characterization of a novel PL7 alginate lyase AlyC3 from Psychromonas sp. C-3 isolated from the Arctic brown alga Laminaria, including its phylogenetic category, catalytic properties, and framework. We suggest the