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Biocompatibility and fibroblast expansion on nanofibers had been investigated. In inclusion, the anti-leishmanial activity of CS-PEO nanofibers both in the promastigote and amastigote stages of was evaluated after parasite important staining and MTT assay and compared to a control group. Analytical analysis had been carried out utilizing SPSS software (version 18.. Statistically significd amastigote stages in vitro. Further studies are required to re