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2-Eu is the first MOF sensor for Mg2+ utilizing the lowest recognition limitation of 1.53 × 10-10 mol L-1 and shows great recyclable capacity. Simultaneously, into the presence of other material ions (Ca2+, Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+, Pd2+, Al3+, Cr3+and Fe3+), 2-Eu can maintain the discerning sensing of Mg2+, showing its possible for Mg2+ turn-on sensing.A novel ligand (6) for copper-catalyzed azide-alkyne cycloaddition (CuAAC) in bioconjugation was developed. Both in vitro plus in