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An effective method to study the active sites for carbocatalysis is proposed based on designing a carbon catalyst in the absence of metal as the growth catalyst. The results suggest that the oxygenated groups on the aromatic carbons are mainly responsible for the catalytic reduction of nitrobenzene and some other reactions.Rapid analysis of metabolites in biofluids is of great importance for disease diagnosis or new-born disease screening. Herein, we introduce an agarose hydrogel conditioning method to enhance the performance of paper sp