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Esophageal biomechanical studies are important to understand structural changes resulting from stretches during repair of esophageal atresias as well as to obtain values to compare with the biomechanics of tissue-engineered esophagus in the future. This study aimed to investigate light microscopic changes after uniaxial stretching of the ovine esophagus. In vitro uniaxial stretching was performed on esophagi (n = 2 of 1-month-old lambs within 4-6h post-mortem. Esophagi were divided into 5 groups control and stretched (1.1, 1.2, 1.3 and