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Remarkably, the loss of your established non-homologous stop signing up for (NHEJ) pathway factors KU70 or perhaps DNA ligase Several (LIG4) fully removed tandem bike duplicate development. The particular microhomology-mediated NHEJ issue POLQ has been essential only for patch-like insertions, which are well-known via DSB restoration because templated insertions coming from ectopic web sites. Since SSBs can be fixed making use of homology, all of us additionally asked whether the traditional homologous recombination process is actually i
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