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The LMs internalization and immune activation of BMDCs were blocked in the presence of cytochalasin, filipin III and chlorpromazine, which demonstrated that internalization of LMs by BMDCs is a key process for the LMs induced immune activation of BMDCs. The results obtained support the further evaluation of LMs as a mycobacterial vaccine, adjuvant, and in immunotherapy. The results obtained support the further evaluation of LMs as a mycobacterial vaccine, adjuvant, and in immunotherapy. This study aimed at highlighting some demographic