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In this research, we developed a general 2D NMR method to differentiate Ganoderma triterpenoids, which categorizes all of them into six kinds (A-F). Then, by the NMR-based separation of A-F type triterpenoids through the fruiting bodies of G. resinaceum, four brand new substances (1-4) and eight understood substances (5-12) had been gotten. Additionally, combined with spiking experiments in 1D and 2D NMR spec
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