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In girls and women, the authors studied the effects of an acute bout of low-impact, moderate-intensity exercise serum on myoblast and osteoblast proliferation in vitro. A total of 12 pre/early pubertal girls (8-10y old) and 12 women (20-30y old) cycled at 60% VO2max for 1hour followed by 1-hour recovery. Blood samples were collected at rest, mid-exercise, end of exercise, mid-recovery, and end of recovery. C2C12 myoblasts and MC3T3E1 osteoblasts were incubated with serum from each time point for 1hour, then monitored for 24hours (myobl