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The use of exercise is a potential treatment option to modulate pain (exercise-induced hypoalgesia). The pain threshold (PT) response is a measure of pain sensitivity that may be a useful marker to assess the effect of physical exercise on pain modulation. The aim of this systematic review and meta-analysis is to evaluate the PT response to exercise in healthy subjects. We searched in MEDLINE, EMBASE, Web of Science, Lilacs, and Scopus using a search strategy with the following search terms "exercise" OR "physical activity" AND "Pain Th