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In this research, three E. coli strains carrying two novel blaNDM variants of blaNDM-36, -37 were identified from someone with refractory endocrine system illness (UTI) in Asia. We carried out antimicrobial susceptibility examination (AST), enzyme kinetics analysis, conjugation research, whole-genome sequencing (WGS), and bioinformatics evaluation to characterize the blaNDM-36, -37 enzymes and their carrying strains. The blaNDM-36, -37 harboring E. col