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All these methods were validated for selectivity, repeatability, and sensitivity. The modified method in this study could not only eliminate the cross-reactivity between antibodies but also monitor the whole procedure of the analysis of EPO with spiked rat EPO. Besides that, rat EPO could also be used as an indicator for monitoring the presence of protease(s) in urine samples.The increasing awareness of sustainability has led to enormous growth of the demand for bio-based and biodegradable polymers such as poly(lactide) (PLA). In industry, polymerization of