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Nevertheless, there is certainly small information on the number preferences and blood-feeding behavior of Anophelinae vectors in outlying Amazonian landscapes. The barrier display sampling (BSS) strategy was employed to test females from 34 peridomestic habitats in 27 rural communities from 11 municipalities within the Brazilian Amazon states of Acre, Amazonas, Pará and Rondônia, from August 2015 to November 2017. Nyssorhynchus darlingi comprised 97.94% of this females