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The aim of the study is to present a photogrammetric technique using standardized occlusal photographs to perform Bolton's analysis and assess reliability of this new method with plaster study casts. The study was conducted on 16 subjects (8 males, 8 females), aged 18-25 years. Standardized occlusal photographs and plaster study casts were obtained. The occlusal photographs were calibrated in Nemoceph® software. Mesio-distal dimensions of all teeth up to first molars were calculated and Bolton's analysis was performed. Similarly, a digi