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The piglets were challenged with an intraperitoneal shot with 100 μg/kg LPS on time 14 to cause abdominal damage. From then on, the gene and necessary protein expression quantities of representative tight junction proteins and myosin light-chain kinase (MLCK)/phosphorylated myosin light cha pretreatment. The in vitro analysis included control, LPS, and GLP-2 + LPS treatments. GLP-2 treatment alleviated the destructive effectation of LPS on buffer permeability by