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O3/H2O2 treated sample reduced the nitrification, especially formation of N - NO 2 - in the first phase of the process. Simultaneously, quadratic model was developed to describe the relationship between oxygen uptake rate and changes in ammonium nitrogen concentration due to the oxidative treatment. The positive impact of ozone was also confirmed by ozonation of Tiamulin-contaminated (400 mg L-1) waste sludge where biogas production potential was increased for 6-times. Combination of approaches confirmed, that O3 effectively increase the