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Objective To investigate the effect of miR-21 on the expression of NF-κB and NLR family pyrin domain containing 3 (NLRP3) in RAW264.7 cells stimulated by lipopolysaccharide (LPS) and its possible mechanism. Methods Real-time fluorescent quantitative PCR was used to detect the expression of miR-21 after RAW264.7 cells were stimulated with 100 ng/mL LPS for 24 hours. miR-21 inhibitors or mimics were transfected to regulate the expression of miR-21 in RAW264.7 cells. After miR-21 inhibitors or mimics were stimulated by LPS, real-time quanti