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Because of this, we employed two fold staining after immunofluorescence for PTP combined with fluorescence probes for the acrosome (PNA-FITC) and PMI (LIVE/DEAD Fixable Dead Cell Stain Kit). Our outcomes unveiled that the presence of PTP at sperm mind was less abundant when you look at the sperm fraction that caused the AE after 3 h of incubation under capacitating conditions, or by its induction with calcium ionophore, when compared to unreacted small fraction. Moreover, PTP in the
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