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Some image marker positions and the smallest image marker (4 × 4cm) led to larger errors in the perceived dimensions of the virtual models than other image marker positions and larger markers (8 × 8 and 12 × 12cm). ≤ 2° and ≤ 2mm errors were found in 70.7% and 76% of cases, respectively. Errors obtained in a non-negligible percentage of cases are not acceptable for certain surgical tasks (e.g. the identification of correct trajectories of surgical instruments). Achieving sufficient accuracy with image marker sizes that meet surgical ne