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In this work, uniform cross-linked chitosan/lignosulfonate (CS/LS) nanospheres with an average diameter of 150-200 nm have been successfully used as a novel, environmentally friendly biocide for the inhibition of mixed sulfate-reducing bacteria (SR culture, thereby controlling microbiologically influenced corrosion (MIC) on carbon steel. It was found that 500 µg·mL-1 of the CS/LS nanospheres can be used efficiently for the inhibition of SRB-induced corrosion up to a maximum of 85% indicated by a two fold increase of charge transfer re