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The aquatic environment is constantly exposed to chemical pollutants from agriculture and the urban environment. In this study, the effects of Bisphenol A, Naphthalene, and Butachlor on metabolic and antioxidant enzymes of goldfish were investigated during subacute exposure. The samples (n = 144, 100.97 ± 31.47 g) were distributed randomly in 12 glass aquaria (70 L) with a stocking density of 12 fish for each. Each aquarium was exposed to one of each pollutant (Bisphenol A with 500 μg/L, Butachlor with 0.28 μL/L and Naphthalene with 200