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There is a positive association between step counts from adults at risk of developing T2DM and their children, especially in the mother-daughter and father-son dyads. There is a positive association between step counts from adults at risk of developing T2DM and their children, especially in the mother-daughter and father-son dyads. This study aimed to analyze the acute effect of physical exercise on nitric oxide concentration and blood pressure (BP) in older adults with different levels of training status (TS) and verified the influence