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e an important therapeutic target for the treatment of DR. The circadian clock plays an important role in the expression and regulation of various genes and cellular processes in the body. Here, we study diurnal regulation of the growth and shedding of the sensory axons in the mouse cornea. Male and female BALB/cN mice were euthanized 90 minutes before and after the lights are turned on and off; at 530 AM, 830 AM, 530 PM, and 830 PM. Nerve terminal growth, shedding and overall axon density were assessed at these four time points using