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iWAT sclerostin content decreased post-exercise in both 28 kDa (-31%, p = 0.04) and 30 kDa groups (-36%, primary impact for exercise, p = 0.02). iWAT β-catenin (+44per cent, p = 0.03) and GSK3β content were greater in HFD mice compared to LFD (+128%, primary result for diet, p = 0.005). Monomeric sclerostin content had been abolished in eWAT of HFD mice (-96%, main effect for diet, p less then 0.0001), was just detectable as a 30 kDa band in LFD mice and was unre