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This study explored the role of calcium nitrate as a bio-stimulant for anaerobic ammonium oxidation (anammox) process. The anaerobic sequencing batch reactor was firstly inoculated with malodorous river sediment and only fed with calcium nitrate until no marked endogenous release of ammonium in effluent (Phase 1). Subsequently, nitrite and ammonium were supplied to test the performance of anammox process (Phase 2). During the operation of Phase 1, the effluent ammonium increased firstly and then decreased. Additionally, continuous nitr