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The results were in good agreement between the new developed method and the referential methods (high-performance liquid chromatography and the conventional plating counts). Copyright © 2020 Ren, Yue, Mwakinyali, Zhang, Zhang and Li.Coronavirus (CoV) envelope (E) protein is a small structural protein critical for virion morphogenesis and release. The recently characterized E protein ion channel activity (EIC) has also been implicated in modulating viral pathogenesis. In this study, we used infectious bronchitis coronaviru