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Lastly, future perspectives and challenges remaining for systems metabolic engineering of E. coli are discussed. Eprinomectin is used as an active pharmaceutical ingredient (API) in various drug products by the animal health industry. Several major related impurities of Eprinomectin API are not separated and coelute by the current United States Pharmacopeia method for Eprinomectin. To develop and validate a true and reliable stability-indicating reversed phase HPLC method for assay and determination of related substances of Eprinomecti