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Increased instantaneous water-use efficiency (IWUE) for the two lower latitudes was primarily attributed to the CO2 stimulation of Pn while the higher IWUE under the photoperiod regimes of 55 and 58°N latitudes was explained by reduced water loss. Photoperiod effects varied with [CO2 ] Pn increased at the photoperiod regimes of 55 and 58°N in ambient [CO2 ] while it tended to decline under these photoperiods in elevated [CO2 ]. Our study suggests that the photosynthesis of white birch will likely respond negatively to northward migration or seed transfer in