https://romidepsininhibitor.co....m/affect-regarding-b
After informed consent, we built-up prospective blood examples from a cohort of 12 melanoma patients with tissue-confirmed BRAF V600E mutation. Each bloodstream specimen was prepared straight away post collection applying two published standard protocols in parallel selecting for EVs and platelets, correspondingly. The RNA of every small fraction ended up being analysed by an extremely painful and sensitive ARMS RT-qPCR enabling the measurement of this mutant allele fraction (%MAF) of BRAF V6
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