https://stenabolic0.com/the-co....operative-understand
Caco-2 cancer cells were routinely cultured with and without treatment with 100 ng/mL EGF, and alterations in mobile morphology had been seen using an inverted microscope. In inclusion, a transwell assay had been used to detect cellular migration beneath the problem of EGF therapy. The phrase of FAK, pFAK, E-cadherin, vimentin, and β actin was examined by western blotting, plus the phrase of miR-217 was assessed using real time PCR. We found that EGF induced EMT in colorectal cancer cells and en