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Conclusion In conclusion, the DIAGNOdent pen shows the highest performance and accuracy to detect smooth surface caries both at D1 and D3 among the three methods. Although VistaCam iX and bitewing radiography showed better performance at D3 compared to D1, their performances were lower than the DIAGNOdent pen.Aim This study evaluated three concentrations of butyl toluidine blue (BuT for antimicrobial photodynamic therapy (aPDT) in experimental periodontitis (EP) in rats. Material and methods EP was ligature-induced at the first mandi