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INTRODUCTION Caffeine and its metabolites have antioxidant activity, scavenging reactive oxygen species. Aim of our study was to measure caffeine concentrations in vitreous samples after peroral caffeine intake. METHODS This prospective study included patients scheduled for 23G pars plana vitrectomy with membrane peeling due to epiretinal membranes. The study was performed in two parts In the first part patients were recruited into three different groups group A consisted of habitual coffee drinkers, that agreed to drink coffee containing