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There has been few scientific studies comparing various preparation and imaging means of imagining the leaf skin. Here, four specimen planning techniques were utilized to investigate the leaf epidermis morphology of Arabidopsis, radish, cucumber, grain, rice, and maize, under an inverted basic light microscope (LM), a laser checking confocal microscope (LSCM), or a scanning electron microscope (SEM). Optical microscope specimens were acquired utilizing either the direct isolation strateg