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This is the first report demonstrating EMCs trophic pathways in situ, constituting an essential baseline for future research and safety regulations involving EMCs in the environment.In this work, a soluble biopolymer was prepared by conjugating the bovine serum albumin (BSA) with transition metal ion (Cu2+). BSA-Cu complex was synthesized and characterized using UV-vis absorption, fluorescence and ATR-FTIR spectroscopies. A colorimetric guaiacol oxidation based method, was used to study the catalytic activity of complex and the results