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Moreover, CIPK1 mutation caused phosphate starvation-induced (PSIs) genes significantly induced under Pi deficiency. Histological staining demonstrated that cipk1 mutant has increased acid phosphatase activity and hydrogen peroxide concentration under Pi deficiency. By using the yeast two-hybrid system, we further demonstrated the interaction between CIPK1 with the WRKY transcription factors WRKY6 and WRKY42. (4) Overall, we demonstrate that CIPK1 is involved in Pi deficiency signaling pathway in Arabidopsis thaliana, revealing the impor