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Their recruitment of additional proteins causes the assembly of a multicomponent NEC, coordinately controlling viral nucleocytoplasmic capsid egress. Here, the functionality of VZV, HCMV, MCMV and EBV core NECs ended up being investigated by coimmunoprecipitation and confocal imaging analyses. Additionally, a recombinant MCMV, harboring an alternative of ORF M50 by UL50, ended up being analyzed in both vitro and in vivo. In essence, core NEC interactions w