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There is no DENND1A.V2 protein equivalent in mice, and also the murine Dennd1a gene is vital for viability since Dennd1a knockout mice are embryonically life-threatening, suggesting that Dennd1a is developmentally important. Three various hDENND1A.V2 transgenic mice lines had been created using CMV, Lhcgr, and TetOn promoters. The hDENND1A.V2 mice expressed hDENND1A.V2 transcripts. While hDENND1A.V2 protein wasn't detectable by Western blot analyses, proper hDENND1A.V2 immunohistochemical staining had been
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