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Compared with the CON group, the value of bone volume/total volume (BV/TV) and trabecular number (Tb.N) of the tibias in the TRE group remarkably decreased ( 0.01). However, these changes were markedly less than those of the T-S group after 4 weeks of EA treatment ( 0.05). Moreover, the serum concentration of BGP in the TRE group was also significantly higher than that of the T-S group ( 0.05). These findings indicate that EA stimulation at BL20, BL23, and SP6 retards osteoporosis induced by hind limb unloading in rats. Thes