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Acid mine drainage (AMD) is one of the most serious environmental problems and extreme environments on the earth, with high concentrations of sulphate and dissolved metals. A comprehensive description of dissolved organic matter (DOM) in these reservoirs is lacking, and it can play an important role in AMD pollution treatment and ecosystem. Thus, the source, composition and property of DOM in an AMD reservoir in Ma'an shan, China were studied using Fourier transform ion cyclotron resonance mass spectrometry and three-dimension excitati