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Cucumber is dioecious by nature having both male and female flowers and is a model system for unisexual flower development. However, knowledge related to male flowering is limited and reported to be regulated by transcription factors and hormone signals. Here, we report functional characterization of the cucumber CsGL2-LIKE gene, which encodes an HD-ZIP IV transcription factor that plays an important role in regulating male flower development. Spatial-temporal expression analyses revealed high-level expression of CsGL2-LIKE in the male fl