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Through detecting trans-epithelial electrical resistance, it was revealed that silencing of PTBP1 broke the integrity of tight junctions between adjacent SCs. The results suggested that PTBP1 maintained the integrity of the BTB by promoting the expression levels of tight junction-associated proteins and revealed the possible mechanism of PTBP1 in regulating spermatogenesis.Osteoprotegerin (OPG) is a member of the tumor necrosis factor receptor superfamily and a major regulatory factor in osteoclast development. OPG has been previously