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This study aimed to evaluate vital pulp tissue removal from different endodontic instrumentation systems from root canal apical third . Thirty mandibular molars were selected and randomly divided into 2 test groups and one control group. Inclusion criteria were a positive response to cold sensibility test, curvature angle between 10 and 20 degrees, and curvature radius lower than 10 mm. Root canals prepared with Hero 642 system (size 45/0.02) ( = 1 and Reciproc R40 (size 40/0.06) ( = 1 and control ( = 1 without instrumentation. Ca